Journal: Frontiers in Cellular Neuroscience

Article Title: Bone Marrow Stromal Cells Alleviate Secondary Damage in the Substantia Nigra After Focal Cerebral Infarction in Rats

doi: 10.3389/fncel.2019.00338

Figure Lengend Snippet: Effects of BMSCs treatment on the number of TH, NeuN, and GFAP positive cells in SN, and DA and its metabolites in striatum at 4 weeks after dMCAO. (A) Representative microphotographs of immunohistochemistry for TH (II,VI,X) , NeuN (III,VII,XI) , and GFAP (IV,VIII,XII) in SN. The pictures on the right are magnified from the square area on the left. Scale bar: I, V, IX, 250 μm; II–IV, VI–VIII, X–XII, 50 μm. (B) Quantitative analyses of TH, NeuN, GFAP-positive cells in the SNc at 1 and 4 weeks after dMCAO. Transplantation of BMSCs increased the numbers of TH + [ F (2, 60) = 31.51, p < 0.01] and NeuN + cells [ F (2, 78) = 17.20, p < 0.01], and decreased the number of GFAP + cells [ F (2, 78) = 1848.10, p < 0.01] in the ipsilateral SNc after dMCAO. Each bar represents the mean ± S.D. * p < 0.05 vs. sham-operated group, # p < 0.05 vs. contralateral groups at the same time point and & p < 0.05 vs. ipsilateral vehicle groups ( n = 6 in each group). (C) The DA (I) , DOPAC (II) , and HVA (III) concentrations in striata at 1 and 4 weeks after dMCAO with or without BMSCs transplantation. Transplantation with BMSCs increased the concentration of DA [ F (2, 8) = 6.33, p < 0.05] in the ipsilateral striatum after dMCAO, maintained the concentration of DOPAC [ F (2, 11) = 0.53, p > 0.05] and HVA [ F (2, 11) = 0.67, p > 0.05] in the ipsilateral striatum after dMCAO. Each bar represents the mean ± SD * p < 0.05 vs. sham-operated group and # p < 0.05 vs. contralateral groups at the same time point and & p < 0.05 vs. ipsilateral vehicle groups ( n = 4 in each group). SN, substantia nigra; SNc, substantia nigra compact part; TH, tyrosine hydroxylase; NeuN, neuron-specific nuclear-binding protein; GFAP, Glial fibrillary acidic protein; Sham, sham-operated; BMSCs, bone marrow stromal cells; DA, dopamine; DOPAC, 3,4-dihydroxyphenylacetic acid; HVA, homovanillic acid; con, contralateral; ip, ipsilateral; w, week; dMCAO, distal middle cerebral artery occlusion.

Article Snippet: Over the past two decades, bone marrow stromal cells (BMSCs) based neurorepair has emerged as a promising therapeutic strategy for ischemic stroke.

Techniques: Immunohistochemistry, Transplantation Assay, Concentration Assay, Binding Assay

Journal: Frontiers in Cellular Neuroscience

Article Title: Bone Marrow Stromal Cells Alleviate Secondary Damage in the Substantia Nigra After Focal Cerebral Infarction in Rats

doi: 10.3389/fncel.2019.00338

Figure Lengend Snippet: Cortico-striatum-nigral tract retrograde tracing with PRV-152. (A) PRV-152 was injected into the ipsilateral SNr. Regions of interest (ROI) of PRV-152 positive cell counting were shown. Dark shaded area represents ischemic region after dMCAO; lighter shaded area represents ROI. (B) Schematic illustration of the fluorescent signal of PRV-152 in sham-operated and dMCAO groups with or without BMSCs transplantation after PRV-152 was injected into the ipsilateral SNr. (C) Representative photographs of fluorescent double staining of PRV-152 (green) and DAPI (blue) in the ipsilateral cortex (I–IV) , striatum (V–VIII) and SNr (IX-XII) at 4 days after PRV-152 injection in sham-operated group. (D) Representative photographs of fluorescent double staining of PRV-152 (green) and DAPI (blue) in the ipsilateral cortex (I–IV) , striatum (V–VIII) , and SNr (IX–XII) at 4 weeks after PRV-152 injection in vehicle group. (E) Representative photographs of fluorescent double staining of PRV-152 (green) and DAPI (blue) in the ipsilateral cortex (I–IV) , striatum (V–VIII) and SNr (IX–XII) at 4 weeks after PRV-152 injection in BMSCs group. Scale bar, 50 μm. (F) Quantitative analyses of PRV-152 + cell number in the ipsilateral cortex, striatum and SNr after dMCAO. Transplantation with BMSCs increased PRV-152 + cells in the ipsilateral cortex [ F (2, 19) = 9.85, p < 0.01], striatum [ F (2, 41) = 10.67, p < 0.01] and SNr [ F (2, 19) = 6.05, p < 0.01] after dMCAO. Each bar represents the mean ± SD * p < 0.05 vs. sham-operated group and # p < 0.05 vs. vehicle group ( n = 7 in each group). SNr, substantia nigra pars reticulata; Cor, cortex; Str, striatum; PRV, pseudorabies virus; Sham, sham-operated; w, week; dMCAO, distal middle cerebral artery occlusion; BMSCs, bone marrow stromal cells; DAPI, 4’, 6- diamidino-2-phenylindole.

Article Snippet: Over the past two decades, bone marrow stromal cells (BMSCs) based neurorepair has emerged as a promising therapeutic strategy for ischemic stroke.

Techniques: Retrograde Tracing, Injection, Cell Counting, Transplantation Assay, Double Staining, Virus